Gene-centromere mapping in meiotic gynogenetic European seabass

Oral M., Colleter J., Bekaert M., Taggart J. B., Palaiokostas C., McAndrew B. J., ...More

BMC GENOMICS, vol.18, 2017 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 18
  • Publication Date: 2017
  • Doi Number: 10.1186/s12864-017-3826-z
  • Journal Name: BMC GENOMICS
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Keywords: Dicentrarchus labrax, Meiotic gynogenesis, Isogenic lines, ddRAD seq, Genetic map, Gene-Centromere map, Aquaculture, MICROSATELLITE LINKAGE MAP, HALF-TETRAD ANALYSIS, TROUT ONCORHYNCHUS-MYKISS, EEL ANGUILLA-JAPONICA, RAINBOW-TROUT, DETERMINATION LOCUS, PROVIDES INSIGHTS, COMMON CARP, DICENTRARCHUS, GENOME
  • Recep Tayyip Erdoğan University Affiliated: Yes

Abstract

Background: Fully isogenic lines in fish can be developed using "mitotic" gynogenesis (suppression of first zygotic mitosis following inactivation of the sperm genome). However, genome-wide verification of the steps in this process has seldom been applied. We used ddRADseq to generate SNP markers in a meiotic gynogenetic family of European seabass (Dicentrarchus labrax): (i) to verify the lack of paternal contribution in a meiotic gynogenetic family; (ii) to generate a gene-centromere map from this family; (iii) to identify telomeric markers that could distinguish mitotic gynogenetics from meiotic gynogenetics, which sometimes arise spontaneously in mitotic gynogenetic families.