Gene-centromere mapping in meiotic gynogenetic European seabass


Oral M. , Colleter J., Bekaert M., Taggart J. B. , Palaiokostas C., McAndrew B. J. , ...More

BMC GENOMICS, vol.18, 2017 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 18
  • Publication Date: 2017
  • Doi Number: 10.1186/s12864-017-3826-z
  • Title of Journal : BMC GENOMICS

Abstract

Background: Fully isogenic lines in fish can be developed using "mitotic" gynogenesis (suppression of first zygotic mitosis following inactivation of the sperm genome). However, genome-wide verification of the steps in this process has seldom been applied. We used ddRADseq to generate SNP markers in a meiotic gynogenetic family of European seabass (Dicentrarchus labrax): (i) to verify the lack of paternal contribution in a meiotic gynogenetic family; (ii) to generate a gene-centromere map from this family; (iii) to identify telomeric markers that could distinguish mitotic gynogenetics from meiotic gynogenetics, which sometimes arise spontaneously in mitotic gynogenetic families.