Purpose: Premature ovarian failure (POF) is the deterioration of normal ovarian function before the age of 40. In the presence of chemotherapy, radiation, genetic factors, autoimmune conditions, hypoandrogenemia, hypoestrogenemia and increased gonadotropin hormones, dry eye with early menopause findings may be encountered. The goal of our study is to compare the tear film alterations and meibomian gland status of patients diagnosed with POF at the time of diagnosis with healthy volunteers. Methods: In our study, 90 patients with POF and 60 control patients were evaluated. Complete ophthalmologic examinations of the patients, ocular surface disease index (OSDI) score, Oxford score for corneal and conjunctival involvement, Schirmer 1 and 2 tests, noninvasive tear break-up time (BUT), lower lid meibomian drop out grades with noncontact meibography, and meibomian gland distortion and shortening scores were compared between the two groups. Results: The mean age was 29.49 ± 2.92 years in the patient group and 29.37 ± 2.85 years in the control group (p = 0.830). OSDI scores were statistically significant higher in the patient group (32.11 ± 18.88) compared to the control group (12.93 ± 14.92) (p < 0.001). On Oxford scoring, there was a significant increase in the patient group (p < 0.001). There was no significant difference between the groups in terms of Schirmer 1 and 2 tests (p = 0.195, p = 0.117). NUBIT was significantly lower in the patient group (11.93 ± 4.59) compared to the control group (18.72 ± 5.38) (Pp < 0.001). While there was no difference between the groups in terms of lower lid meiboscores or meibomian gland length (p > 0.005), there was a significant deterioration in the patient group in the distortion grading showing the morphological evaluation of the meibomian glands (p = 0.037). In the ROC analysis, OSDI score (AUC = 0.816,p < 0.0001) and NIBUT (AUC = 0.820, p < 0.0001) parameters showed high specifity and sensitivity for the disease. Discussion: Ocular surface damage and dry eye symptoms are observed more frequently in patients with POF. We believe that hormonal insufficiency may cause deterioration in tear film composition, ocular surface damage with changes in tear homeostasis, and a change in the structure of the meibomian glands, starting with distortion at an early age.