The Role of Antioxidant Activity in the Prevention and Treatment of Infertility Caused by Cisplatin in Rats


Aksoy A. N. , Kucur S. K. , Batmaz G., Gozukara I., AKSOY M., Kurt N., ...Daha Fazla

GYNECOLOGIC AND OBSTETRIC INVESTIGATION, cilt.79, sa.2, ss.119-125, 2015 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 79 Konu: 2
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1159/000367657
  • Dergi Adı: GYNECOLOGIC AND OBSTETRIC INVESTIGATION
  • Sayfa Sayıları: ss.119-125

Özet

Background/Aims:To investigate the importance of antioxidant activity in infertility caused by cisplatin in rats. Methods: Rats in cisplatin control (CG), Vitamin E+cisplatin (ECG), Vitamin C + cisplatin (CCG), Hippophae rhamnoides extract (HRE) + cisplatin (HRECG), and thiamine pyrophosphate (TPP) + cisplatin (TPPCG) groups were injected intraperitoneally (ip) with (100 mg/kg) Vitamin E, Vitamin C, HRE, and TPP, respectively. One hour later, ip cisplatin was administered (5 mg/kg), and then antioxidant medications were continued for 10 days. Cisplatin + Vitamin E (CEG-1), cisplatin + Vitamin C (CCG-1), cisplatin + HRE (CHREG-1), and cisplatin + TPP (TPPCG-1) rats received cisplatin (5 mg/kg, ip) and were kept for 10 days. At the end of that period, rats received antioxidant medications for 10 days. (n = 12, for each group). Six rats from each group were sacrificed. Ovaries were removed to measure malondialdehyde, total glutathione, glutathione S-transferase, and glutathione reductase levels. The remaining rats were kept in a suitable laboratory environment. Results: Cisplatin-induced oxidative stress was best prevented by HRE, Vitamin E, Vitamin C, and TPP, in that order. However, infertility caused by cisplatin was only prevented and treated by TPP. Conclusion: Oxidative stress is not a major component in the pathogenesis of cisplatin-associated infertility. (C) 2015 S. Karger AG, Basel