Akademik Veri Yönetim Sistemi
14 th International Symposium on Pharmaceutical Sciences (ISOPS-14), Ankara, Türkiye, 25 - 28 Haziran 2024, ss.1
The isolation and evaluation of the biological activity of naturally occurring
compounds is one of the major topics in pharmaceutical sciences. The genus Scorzonera has
approximately 180 species in the world and 52 species, 31 of which are endemic, grow
naturally in Turkey. Scorzonera L. species have been reported to be consumed as food and
used in traditional medicine (1,2). Phytochemical studies on Scorzonera species revealed that
the genus is rich in flavonoids, dihydroisocoumarins, triterpenes and sesquiterpenes (3,4). As
part of an ongoing study on the Scorzonera genus, a phytochemical analysis was conducted
on Scorzonera hieraciifolia species grown in Turkey.
Materials and Methods: S. hieraciifolia was collected from Konya in June 2009 [Herbarium
No: Makbul 127 & Çoşkunçelebi (RUB)]. The underground tubers of the plant were extracted
with methanol according to the maceration method and the crude methanol extract was
fractionated with n-hexane, dichloromethane, n-butanol, and water. Chromatographic
separation of n-hexane and n-butanol fractions of the crude methanol extract obtained from
the underground tubers of S. hieraciifolia led to the isolation of 6 pure compounds.
Results: A total of 6 compounds from the n-hexane and n-butanol fractions of S. hieraciifolia;
three triterpenes (taraxasterol, pseudotaraxasterol and taraxasterol-3-O-β-acetate), a new
flavone heteroside (scorzohiearacoside), carbohydrate (sucrose), and 1-octadecene were
isolated by various chromatographic methods. Their structures have been elucidated and
ascertained spectroscopically (NMR 1H, 13C, COSY, HMBC, HSQC, and LC-QTOF-MS).
Conclusions: Scorzohiearacoside, a novel flavonoside, was detected for the first time in this
study. All other compounds were isolated from S. hieraciifolia for the first time. The isolated
pure compounds have the potential to contribute both to the chemotaxonomy of the genus and
to drug discovery studies with pharmacological activity studies to be carried out on the
compounds to be isolated.