The synthesis of new hybrid compounds containing several heterocyclic groups namely norfloxacine, 1,2,4-triazole, 1,3,4-oxadiazole, piperazine, morpholine, thiomorpholine, 1,3,4-thiadiazole etc. was performed by conventional and successfully optimized microwave assisted techniques. The effect of acid catalyst on one pot tricomponent Mannich reaction was investigated as well. The structures of newly synthesized compounds were eluciated on the basis of spectroscopic techniques. Also, the synthesized compounds were screened for their antimicrobial activities and most of them were found to possess good-moderate antimicrobial activity.