Characterization of novel VIM carbapenemase, VIM-38, and first detection of GES-5 carbapenem-hydrolyzing beta-lactamases in Pseudomonas aeruginosa in Turkey

Iraz, Meryem; Duzgun, Azer; Cicek, AYŞEGÜL; BONNIN, Remy; Ceylan, Aysenur; Saral, Aysegul; NORDMANN, Patrice; SANDALLI, CEMAL

doi:10.1016/j.diagmicrobio.2013.12.003

Characterization of novel VIM carbapenemase, VIM-38, and first detection of GES-5 carbapenem-hydrolyzing beta-lactamases in Pseudomonas aeruginosa in Turkey

Atıf İçin Kopyala

Iraz M., Duzgun A. O., Cicek A., BONNIN R. A., Ceylan A., Saral A., ...Daha Fazla

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, cilt.78, sa.3, ss.292-294, 2014 (SCI-Expanded)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 78 Sayı: 3
Basım Tarihi: 2014
Doi Numarası: 10.1016/j.diagmicrobio.2013.12.003
Dergi Adı: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.292-294
Recep Tayyip Erdoğan Üniversitesi Adresli: Evet

Özet

Pseudomonas aeruginosa isolates were collected form a Turkish hospital. Antimicrobial susceptibility was performed using the Vitek 2 Compact system, and 24 isolates were categorized as multidrug resistant (n = 18), extensively-drug resistant (n = 5), or pan-drug resistant (n = I). PCR and DNA sequence analysis revealed that 1 strain possessed the bla(GES-5) and another carried a novel bla(VIM) variant, named VIM-38. This new gene exhibited 1 amino acid substitution (Ala265Val) in comparison to its closest variant, VIM-5. Both VIM encoding genes were clones and demonstrated similar susceptibility profile when expressed in identical background. The presence of VIM-38 increases the diversity of carbapenemases in Turkey. (C) 2014 Elsevier Inc. All rights reserved.