Characterization of novel VIM carbapenemase, VIM-38, and first detection of GES-5 carbapenem-hydrolyzing beta-lactamases in Pseudomonas aeruginosa in Turkey


Iraz M., Duzgun A. O. , Cicek A. , BONNIN R. A. , Ceylan A., Saral A., ...More

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, vol.78, no.3, pp.292-294, 2014 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 78 Issue: 3
  • Publication Date: 2014
  • Doi Number: 10.1016/j.diagmicrobio.2013.12.003
  • Title of Journal : DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
  • Page Numbers: pp.292-294

Abstract

Pseudomonas aeruginosa isolates were collected form a Turkish hospital. Antimicrobial susceptibility was performed using the Vitek 2 Compact system, and 24 isolates were categorized as multidrug resistant (n = 18), extensively-drug resistant (n = 5), or pan-drug resistant (n = I). PCR and DNA sequence analysis revealed that 1 strain possessed the bla(GES-5) and another carried a novel bla(VIM) variant, named VIM-38. This new gene exhibited 1 amino acid substitution (Ala265Val) in comparison to its closest variant, VIM-5. Both VIM encoding genes were clones and demonstrated similar susceptibility profile when expressed in identical background. The presence of VIM-38 increases the diversity of carbapenemases in Turkey. (C) 2014 Elsevier Inc. All rights reserved.