Characterization of novel VIM carbapenemase, VIM-38, and first detection of GES-5 carbapenem-hydrolyzing beta-lactamases in Pseudomonas aeruginosa in Turkey

Iraz, Meryem; Duzgun, Azer; Cicek, AYŞEGÜL; BONNIN, Remy; Ceylan, Aysenur; Saral, Aysegul; NORDMANN, Patrice; SANDALLI, CEMAL

doi:10.1016/j.diagmicrobio.2013.12.003

Characterization of novel VIM carbapenemase, VIM-38, and first detection of GES-5 carbapenem-hydrolyzing beta-lactamases in Pseudomonas aeruginosa in Turkey

Iraz M., Duzgun A. O., Cicek A., BONNIN R. A., Ceylan A., Saral A., ...Daha Fazla

DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, cilt.78, sa.3, ss.292-294, 2014 (SCI-Expanded, Scopus)

Yayın Türü: Makale / Tam Makale
Cilt numarası: 78 Sayı: 3
Basım Tarihi: 2014
Doi Numarası: 10.1016/j.diagmicrobio.2013.12.003
Dergi Adı: DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
Sayfa Sayıları: ss.292-294
Recep Tayyip Erdoğan Üniversitesi Adresli: Evet

Özet

Pseudomonas aeruginosa isolates were collected form a Turkish hospital. Antimicrobial susceptibility was performed using the Vitek 2 Compact system, and 24 isolates were categorized as multidrug resistant (n = 18), extensively-drug resistant (n = 5), or pan-drug resistant (n = I). PCR and DNA sequence analysis revealed that 1 strain possessed the bla(GES-5) and another carried a novel bla(VIM) variant, named VIM-38. This new gene exhibited 1 amino acid substitution (Ala265Val) in comparison to its closest variant, VIM-5. Both VIM encoding genes were clones and demonstrated similar susceptibility profile when expressed in identical background. The presence of VIM-38 increases the diversity of carbapenemases in Turkey. (C) 2014 Elsevier Inc. All rights reserved.