The composition of essential oils of Rindera lanata (LAM). Bunge var. canescens (A.D.C) Kosn. obtained by hydro-distillation (HD) and microwave assisted distillation (MW) by GC, GC/MS. Thirty three and thirty nine compounds were identified in the oils representing 81.20% and 78.57% of the oils obtained by HD and MW respectively. Aldehydes were shown to be the main group of constituents of the MW 40.11% and 15.23%, respectively. However, the major group in the HD was found to be 25.35% alcohols and 23.78% hydrocarbons. 6-Methyl heptan-2-ol (15.97%) was the main compound of the HD. Furthermore, in the MW assisted essential oil, the major compound present was decane (10.50%). Terpenoid class compounds were found in essential oils and oxygenated monoterpenes were determined as major group (13.34% and 7.19%). Antimicrobial activity of the isolated essential oils of the plant was also investigated and they showed moderate antimicrobial activity against the tested microorganisms.