Determination of a novel integron-located variant (bla(OXA-320)) of Class D beta-lactamase in Proteus mirabilis


Cicek A. , Duzgun A. O. , Saral A., Sandalli C.

JOURNAL OF BASIC MICROBIOLOGY, cilt.54, ss.1030-1035, 2014 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Cilt numarası: 54 Konu: 10
  • Basım Tarihi: 2014
  • Doi Numarası: 10.1002/jobm.201300264
  • Dergi Adı: JOURNAL OF BASIC MICROBIOLOGY
  • Sayfa Sayıları: ss.1030-1035

Özet

Proteus mirabilis (P. mirabilis) is one of Gram-negative pathogens encountered in clinical specimens. A clinical isolate (TRP41) of P. mirabilis was isolated from a Turkish patient in Turkey. The isolate was identified using the API 32GN system and 16S rRNA gene sequencing and it was found resistant to ampicillin/sulbactam, piperacillin, tetracycline, and trimethoprim/sulfamethoxazole. This isolate was harboring a Class 1 integron gene cassette and its DNA sequence analysis revealed a novel bla(OXA) variant exhibiting one amino acid substitution (Asn266Ile) from bla(OXA-1). This new variant of OXA was located on Class 1 integron together with aadA1 gene encoding aminoglycoside-modifying enzymes. According to sequence records, the new variant was named as bla(OXA-320). Cassette array and size of integron were found as bla(OXA-320)-aadA1 and 2086bp, respectively. The bla(OXA-320) gene is not transferable according to conjugation experiment. In this study, we report the first identification of bla(OXA-320)-aadA1 gene cassette, a novel variant of Class D -lactamase, in P. mirabilis from Turkey.