Topiramate ameliorates abdominal aorta cross-clamping induced liver injury in rats

Cure E., Cure M. C., Tumkaya L., Kalkan Y., Aydin I., Kirbas A., ...More

SAUDI JOURNAL OF GASTROENTEROLOGY, vol.20, no.5, pp.297-303, 2014 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 20 Issue: 5
  • Publication Date: 2014
  • Doi Number: 10.4103/1319-3767.141690
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.297-303
  • Keywords: Asymmetric dimethyl arginine, carbonic anhydrase II, ischemia reperfusion, liver, topiramate, NITRIC-OXIDE, ISCHEMIA/REPERFUSION INJURY, ANTIEPILEPTIC DRUGS, HEPATIC ISCHEMIA, ANTIOXIDANT, DERIVATIVES, MELATONIN, PROTECTS, ARGININE, DAMAGE
  • Recep Tayyip Erdoğan University Affiliated: Yes


Background and Aim: Ischemia/reperfusion (I/R) injury in the liver occurs after a prolonged period of ischemia followed by restoration of hepatic blood perfusion. During the surgery of abdominal aorta, I/R injury causes damage to lower extremities and many organs, especially liver. The antioxidant and tumor necrosis factor-alpha (TNF-alpha) suppression effects of topiramate (TPM) have been reported in several studies. We evaluated the potential protective effect of TPM on cellular damage in liver tissue during I/R injury. Materials and Methods: Thirty male Wistar albino rats were divided into three groups: Control, I/R, and I/R plus TPM (I/R + TPM) groups. Laparotomy without I/R injury was performed in the control group. After laparotomy, cross-ligation of infrarenal abdominal aorta was applied for 2 h in I/R groups that was followed by 2 h of reperfusion. TPM (100 mg/kg/day) was orally administrated to the animals in the I/R + TPM group for seven consecutive days before I/R procedure. Results: The I/R group's TNF-alpha and interleukin-6 (IL-6) levels were significantly higher than those of the control (P = 0.010; P = 0.002) and I/R + TPM groups (P = 0.010; P = 0.002, respectively). Asymmetric dimethyl arginine (ADMA) levels of I/R group were higher than the control (P = 0.015) and I/R + TPM groups. I/R caused serious histopathological damage to liver tissue; however, TPM led to very low histopathological changes. Conclusion: Our data demonstrated that TPM treatment prominently decreases the severity of liver I/R injury. TPM pretreatment may have preventive effects on liver injury via I/R during intra-abdominal surgery.