We present the whole-rock and the mineral chemical data for upper mantle peridotites from the Harmancik region in NW Turkey and discuss their petrogenetic-tectonic origin. These peridotites are part of a Tethyan ophiolite belt occurring along the Izmir-Ankara-Ercincan suture zone in northern Turkey, and include depleted lherzolites and refractory harzburgites. The Al2O3 contents in orthopyroxene and clinopyroxene from the depleted lherzolite are high, and the Cr-number in the coexisting spinel is low falling within the abyssal field. However, the orthopyroxene and clinopyroxene in the harzburgites have lower Al2O3 contents for a given Cr-number of spinel, and plot within the lower end of the abyssal field. The whole-rock geochemical and the mineral chemistry data imply that the Harmancik peridotites formed by different degrees of partial melting (similar to%10-27) of the mantle. The depleted lherzolite samples have higher MREE and HREE abundances than the harzburgitic peridotites, showing convex-downward patterns. These peridotites represent up to similar to 16 % melting residue that formed during the initial seafloor spreading stage of the Northern Neotethys. On the other hand, the more refractory harzburgites represent residues after similar to 4-11 % hydrous partial melting of the previously depleted MOR mantle, which was metasomatized by slab-derived fluids during the early stages of subduction. The Harmancik peridotites, hence, represent the fragments of upper mantle rocks that formed during different stages of the tectonic evolution of the Tethyan oceanic lithosphere in Northern Neotethys. We infer that the multi-stage melting history of the Harmancik peridotites reflect the geochemically heterogeneous character of the Tethyan oceanic lithosphere currently exposed along the Izmir-Ankara-Erzincan suture zone.