Bioremediation is widely used to remove water pollution as environmentally friendly smart solutions. In this study, Bacillus isolates were investigated in terms of the effectiveness of single and multiple cultures in eliminating aquatic pollution related to aquaculture activities. In the established experimental setups, the environments where Bacillus isolates were inoculated with single and multiple cultures at 1 x 10(7) CFU/mL were evaluated comparatively with control groups without these isolates, and total aerobic mesophilic bacterial counts were performed in the petri dish by inoculation method. At the end of the 6 days of the experiment, in the environment in which single and multiple cultures of Bacillus isolates were presented with 17-20 +/- 0.05 degrees C temperature and 5.1-8.1 pH 2-4.6 mg/l dissolved oxygen values (O-2), 2% increase in total phosphorus (TP) value was observed. On the other hand, 4% removal of Ammonia-nitrogen (NH3-N), 80% removal of Nitrite-nitrogen (NO2-N), and 100% removal of Nitrate-nitrogen (NO3-N) were observed. In the changes in heavy metal concentrations, the removal of Ni, Cr, Se, Al, Cd, Mn, Fe, and B was observed from highest to lowest as 57%, 50%, 50%, 43%, 40%, 23%, 5%, and 2%, respectively. It also has been seen that B. thuringiensis isolate was observed to be more effective than B.subtilis in metal removal.