Increased oxidant stress and decreased antioxidant status in erythrocytes of rats fed with zinc-deficient diet

TAYSI S., CIKMAN O., Kaya A., Demircan B., Gumustekin K., Yilmaz A., ...Daha Fazla

BIOLOGICAL TRACE ELEMENT RESEARCH, cilt.123, ss.161-167, 2008 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 123
  • Basım Tarihi: 2008
  • Doi Numarası: 10.1007/s12011-008-8095-x
  • Dergi Adı: BIOLOGICAL TRACE ELEMENT RESEARCH
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.161-167
  • Anahtar Kelimeler: lipid peroxidation, antioxidant enzymes, zinc, free radical, antioxidant potential, oxidative stress, nitric oxide, SERUM OXIDANT/ANTIOXIDANT STATUS, SUPEROXIDE-DISMUTASE ACTIVITY, LIPID-PEROXIDATION, GLUTATHIONE-PEROXIDASE, OXIDATIVE STRESS, NITRIC-OXIDE, EXERCISE, SUPPLEMENTATION, MECHANISMS, REDUCTASE
  • Recep Tayyip Erdoğan Üniversitesi Adresli: Hayır

Özet

The aim of this study was to evaluate the lipid peroxidation, nitric oxide (NO), and free radical scavenging enzyme activities in erythrocytes of zinc (Zn)-deficient rats and to investigate the relationship among these parameters in either group. Sixteen male rats with a weight of 40-50 g were used for the experiment. The rats were divided into control (n=8) and Zn-deficient groups. At the end of the experiment, the animals were anesthetized with ketamine-HCl (Ketalar, 20 mg/kg(-1), i.p.), and the blood was collected by cardiac puncture after thoracotomy. Blood samples were collected in vacutainer tubes without and with K(3)-EDTA as anticoagulant. Erythrocyte catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GRD), glutathione-S-transferase (GST), superoxide dismutase (SOD) activities, total (enzymatic plus nonenzymatic) superoxide scavenger activity (TSSA), nonenzymatic superoxide scavenger activity (NSSA), antioxidant potential (AOP), and serum zinc (Zn) values in the Zn-deficient group were significantly lower than those of the control group, whereas NO and malondialdehyde (MDA) levels were significantly higher than those of the control group. The results show that Zn deficiency causes a decrease in antioxidant defense system and an increase in oxidative stress in erythrocyte of rats.