Akademik Veri Yönetim Sistemi
JOURNAL OF BIOTECHNOLOGY AND STRATEGIC HEALTH RESEARCH, cilt.3, sa.2, ss.111-116, 2019 (Hakemli Dergi)
Objective: The aim of this study was to evaluate the antiproliferative activities of some the fruits, traditionally used in folk medicine. Therefore, the fruits of Fructus cynosbati (Rosehip), Rubus fruticosus (Blackberry), Sorbus torminalis (Wild service tree), Vaccinium myrtillus (Blueberry) and Vitis labrusca (Isabella grape) were used in the present study.
Materials and Methods: Methanol extracts of the fruits were obtained and added to ARPE-19 (no-trasformed) and HeLa (transformed) culture media at various concentrations. Taxol and DMSO were used as positive and negative controls, respectively. After 48 h of cell incubation, the cytotoxic effect of the extracts was assessed by MTT. Cell viability values were calculated for each group. The statistical analysis of the data was performed using unpaired t-test. p values less than 0.05 were considered significant.
Results. V.labrusca showed dose dependent cytotoxic activity against both cell lines. In additions, V.myrtillus and R.fruticosus extracts exhibited moderate cytotoxic effect on both cell lines at higher concentrations. Extracts of F.cynosbati and S.torminalis were found to decrease the cell viability in a dose-dependent manner in HeLa cells and the results were statistically significant (p <0.05). Additionally, S.torminalis extract was found to be the most effective extract against the HeLa cells.
Conclusion: These results demonsrated that S.torminalis methanol extracts has a potent in vitro cytotoxic effect toward the human cervical cancer cell line HeLa. Further work needs to be done on isolation and purification of specific bioactive compounds in S.torminalis methanol extract.