Does different substrates affectantioxidant properties and antimicrobial activity of Pleurotus ostreatus?”

PEKŞEN A., ALPAY KARAOĞLU Ş., Sibel A., Can Z., Arif B.

1th International Eurasia Mycology Congress to be held in Manisa, 3 - 05 July 2017

  • Publication Type: Conference Paper / Summary Text
  • Recep Tayyip Erdoğan University Affiliated: Yes


Does different substrates affect antioxidant properties and
antimicrobial activity of Pleurotus ostreatus?
Aysun PEKŞEN1, Şengül Alpay KARAOĞLU2, Sibel AVCI2, Zehra CAN3, Arif
1Ondokuz Mayis University, Faculty of Agriculture, Department of Horticulture, Samsun, Turkey
2Recep Tayyip Erdoğan University, Faculty of Science and Arts, Department of Biology, Rize, Turkey
3Giresun University, Şebinkarahisar Technical Sciences Vocational School, Turkey
Antioxidant properties and antimicrobial activities of Pleurotus ostreatus grown
on different tree sawdust including populus (PS), fagus (FS), quercus (QS), tilia (TS)
and alnus (AS) alone and with 20% supplementation of tea waste (TW) and wheat
bran (WB) and on logs of populus, fagus and alnus and also collected from nature
were compared in the present study.

A total of 19 P. ostreatus samples were used to determine antioxidant
properties and antimicrobial activities. Antimicrobial activities of methanolic extracts
from P. ostreatus against to Escherichia coli, Yersinia pseudotuberculosis,
Pseudomonas aeruginosa, Enterococcus faecalis, Listeria monocytogenes,
Staphylococcus aureus, Bacillus cereus, Mycobacterium smegmatis, Candida
albicans and Saccharomyces cerevisiae were tested by agar well methods.
Ampicilline (10 μg) and fluconazole (5 μg) were used as the standard control agents
for bacteria and yeasts, respectively. The total phenolic content was measured using
the Folin-Ciocalteau procedure. The antioxidant capacity was determined using ferric
reducing antioxidant power (FRAP) and free radical scavenging activity of DPPH.
It was determined that the extract of mushrooms obtained from the
80QS+20TW substrate was the most effective antimicrobial agent against to all of
bacteria and yeasts investigated in the present study. Significant differences (P<0.01)
were found among the antioxidant contents of the mushroom extracts obtained from
different substrates. Total phenolic, ferric reducing antioxidant power (FRAP) and
scavenging of free radical (DPPH) assay contents of methanolic extracts from P.
ostreatus varied between 1.016 and 4.772 mg GAE/g, 2.245 and 8.902 μmol
FeSO4.7H2O/g, 4.650 and 22.922 mg/mL, respectively.
According to the study results, extracts obtained from P. ostreatus which
grown on different substrates had significant antioxidant activity.

Keywords: Mushroom, antimicrobil, antioxidant, phenolics