Characterization and inhibition studies of carbonic anhydrase from gill of Russian Sturgeon Fish (Acipenser gueldenstaedtii)

Dincer B., Ekinci A. P., AKYUZ G., Kurtoglu I. Z.

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, vol.31, no.6, pp.1662-1665, 2016 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 31 Issue: 6
  • Publication Date: 2016
  • Doi Number: 10.3109/14756366.2015.1076810
  • Journal Name: JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.1662-1665
  • Keywords: Acipenser gueldenstaedtii, carbonic anhydrase, Russian Sturgeon, gill, TROUT ONCORHYNCHUS-MYKISS, KINETIC-PROPERTIES, AMMONIA, PURIFICATION, ACTIVATORS, TARGETS, LIVER, IX
  • Recep Tayyip Erdoğan University Affiliated: Yes

Abstract

An alpha-carbonic anhydrase (CA, EC 4.2.1.1) was purified and characterized kinetically from gill of Acipenser gueldenstaedtii as an endangered sturgeon species. The carbonic anhydrase was purified 66-folds with yield 20.7% by Sepharose-4B-L-tyrosine-sulfanilamide affinity column and the specific activity was determined as 222.2 EU/mg protein. K-m and V-max kinetic values for gill carbonic anhydrase were calculated by a Lineweaver-Burk graph using p-nitrophenol acetate (p-NPA) as a substrate, and was defined as 2.5mM and 5 x 10(6) mu M/min, respectively. It was observed that CA from the sturgeon gill in the presence of the sulfanilamide and acetazolamide as an inhibitor had very low IC50 values such as 13.0 and 0.1 mu M, respectively. In addition, it was determined that the enzyme was inhibited by Fe-2+,Fe- Co-2+,Co- Ni2+, and Zn2+-Ba2+ with the IC50 values of 0.2, 1.7, 1.2, and 1.1 mM, respectively.