Effect of Antioxidants on Cryopreserved Turbot (Scophthalmus maximus) Spermatozoa Quality and DNA Damage


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Polat H., Kurtoglu I. Z.

TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES, vol.23, no.4, 2023 (SCI-Expanded) identifier identifier

  • Publication Type: Article / Article
  • Volume: 23 Issue: 4
  • Publication Date: 2023
  • Doi Number: 10.4194/trjfas22300
  • Journal Name: TURKISH JOURNAL OF FISHERIES AND AQUATIC SCIENCES
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Aqualine, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Veterinary Science Database
  • Keywords: Antioxidant, Cryopreservation, DNA damage, Sperm quality, Turbot, TROUT ONCORHYNCHUS-MYKISS, SPERM MOTILITY, FERTILIZING ABILITY, PSETTA-MAXIMA, AMINO-ACIDS, TAURINE, EXTENDER, HYPOTAURINE, PERFORMANCE, IMPROVE
  • Recep Tayyip Erdoğan University Affiliated: Yes

Abstract

The effects of several antioxidants added to cryopreservation extenders on turbot (Scophthalmus maximus) sperm post-thaw motility, fertilization, sperm characteristics, and DNA damage were investigated. The following antioxidants were used in conjunction with the conventional extenders: rosmarinic acid (RA) (25, 50, and 100 mu g/ml); uric acid (UA) ( 25, 50, and 100 mu g/ml); and taurine (TA) (25, 50, and 100 mu g/ml). On sperm samples that had been diluted by the extenders at a 1:9 ratio, cryopreservation was carried out. The results demonstrated that the post-thaw motility of sperm was higher in 25 mu g/ml RA (77.41 +/- 2.90%), followed by 50 mu g/ml RA (74.86 +/- 3.2%), 50 and 100 mu g/ml UA (69.56 +/- 3.30% and 70.74 +/- 2.51%, respectively) which significantly differentiated from conventional extender. The highest hatching rate (70.67 +/- 4.67%) was attained at UA 50 mu g/ml, while the highest fertilization rate (81.67 +/- 2.52%) was observed in RA 25 mu g/ml. At UA 50 mu g/ml, the DNA fragmentation rate was lowest (5.07 +/- 1.57%). In conclusion, antioxidants had a protective impact on sperm of turbot that had been frozen and thawed.