A novel model approach for esophageal burns in rats: A comparison of three methods


Kalkan Y., Tumkaya L. , Akdogan R. A. , Yucel A. F. , TOMAK Y., Sehitoglu I., ...Daha Fazla

TOXICOLOGY AND INDUSTRIAL HEALTH, cilt.31, sa.7, ss.595-601, 2015 (SCI İndekslerine Giren Dergi) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 31 Konu: 7
  • Basım Tarihi: 2015
  • Doi Numarası: 10.1177/0748233713480208
  • Dergi Adı: TOXICOLOGY AND INDUSTRIAL HEALTH
  • Sayfa Sayıları: ss.595-601

Özet

Background: Corrosive esophageal injury causes serious clinical problems. We aimed to create a new experimental esophageal burn model using a single catheter without a surgical procedure. Materials and methods: We conducted the study with two groups of 12 male rats that fasted for 12 h before application. A modified Foley balloon catheter was inserted into the esophageal lumen. The control group was given 0.9% sodium chloride, while the experimental group was given 37.5% sodium hydroxide with the other part of the catheter. After 60s, esophagus was washed with distilled water. The killed rats were examined using histopathological methods after 28 days. Results: In comparison with the histopathological changes experienced by the study groups, the control groups were observed to have no pathological changes. Basal cell degeneration, dermal edema, and a slight increase in the keratin layer and collagen density of submucosa due to stenosis were all observed in the group subjected to esophageal corrosion. Conclusion: A new burn model can thus, we believe, be created without the involvement of invasive laparoscopic surgery and general anesthesia. The burn in our experiment was formed in both the distal and proximal esophagus, as in other models; it can also be formed optionally in the entire esophagus.