The effect of endothelin receptor antagonists in the endotoxin-induced uveitis rabbit model

Karaca E. E., Uzun F., DİLEKÖZ E., Fincan G. S. O., ERCAN S., KUL O., ...More

CUTANEOUS AND OCULAR TOXICOLOGY, vol.37, no.2, pp.133-138, 2018 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 37 Issue: 2
  • Publication Date: 2018
  • Doi Number: 10.1080/15569527.2017.1355317
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.133-138
  • Keywords: Uveitis, lipopolysaccharides, endothelin receptors, endothelin, ETA-RECEPTOR, POTENT, EXPRESSION, RAT, PEPTIDES, BOSENTAN, DISEASE, SYSTEM, CELLS, EYE
  • Recep Tayyip Erdoğan University Affiliated: Yes


Purpose: To investigate the effect of Bosentan (non-selective endothelin receptor antagonist) and BQ123 (ETA receptor antagonist) on intraocular inflammation in an endotoxin-induced uveitis (EIU) rabbit model.Methods: Uveitis was induced by intravitreal injection of lipopolysaccharide (LPS). The animals were divided into 7 groups and there were six rabbits in each group (saline, saline and ethanol, bosentan, BQ123, lipopolysaccharide (LPS), bosentan and LPS, BQ123 and LPS-injected groups). Bosentan and BQ123 were applied before LPS injection. Aqueous humour was collected at 24th hour post-injections and enucleation was performed for the evaluation of histopathological changes.Results: BQ123 decreased clinical score, cell counts and protein amount more than bosentan and it was significant for cell counts (p=0.018). Bosentan significantly diminished inflammatory reactions more than BQ123 as shown in histopathological specimens (p=0.002).Conclusions: ETA receptor blockage is effective on uveitis treatment by its protective effect on blood aqueous barrier.