The nemacheilid genus Seminemacheilus is revised. Six species are recognised, three of them described herein as new. All species arc endemic to Central Anatolia. Seminemacheilus lendlii from the Sakarya River drainage and the cndorheic Lake Aksehir and Eber basins, S. ispartensis from Lake Egirdir basin and S. ahmeti from Sultan Sazligi are valid species. Although Seminemacheilus lendlii and S. ispartensis have almost identical COI sequences, they are distinguished by the shape of the caudal peduncle and the presence of scales on the caudal peduncle in S. ispartensis (vs. absent in S. lendlii). Seminemacheilus attalicus, new species, from Kirkgoz drainage, is distinguished by having a slightly emarginated caudal fin, a central pore in the supratemporal canal and a marbled flank pattern. Seminemacheilus elcmekciae, new species, from Lake Tuz basin is distinguished by having a roundish caudal fin, a short post-dorsal length and large brown blotches fused into stripes on the flank. Seminemacheilus tubae, new species, from Lake Beyehir basin, is distinguished by having a truncate caudal fm and 2-5 (6) supraorbital head pores. Seminemacheilus dursunavsari from the GOksu River drainage is not a valid name and this population is identified as S. tubae.