Effect of the Toll-Like Receptor 4 Antagonist Eritoran on Retinochoroidal Inflammatory Damage in a Rat Model of Endotoxin-Induced Inflammation


Ekici F. , Karaca E. E. , KORKMAZ S., Yuksel O., Gulbahar O., ALPER M., ...More

MEDIATORS OF INFLAMMATION, vol.2014, 2014 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 2014
  • Publication Date: 2014
  • Doi Number: 10.1155/2014/643525
  • Title of Journal : MEDIATORS OF INFLAMMATION

Abstract

Purpose. We investigated the effect of eritoran, a Toll-like receptor 4 antagonist, on retinochoroidal inflammatory damage in an endotoxin-induced inflammatory rat model. Methods. Endotoxin-induced inflammatory model was obtained by intraperitoneal injection of 1.5mg/kg lipopolysaccharide (LPS). Group 1 had control rats; in groups 2-3 LPS and 0.5mg/kg sterile saline were injected; and in groups 4-5 LPS and 0.5mg/kg eritoran were injected. Blood samples were taken and eyes were enucleated after 12 hours (h) (groups 2 and 4) or 24 hours (Groups 3 and 5). Tumor necrosis factor-alpha (TNF-alpha) and malondialdehyde (MDA) levels in the serum and retinochoroidal tissue and nuclear factor kappa-B (NF kappa B) levels in retinochoroidal tissue were determined. Histopathological examination was performed and retinochoroidal changes were scored. Results. Eritoran treatment resulted in lower levels of TNF-alpha, MDA, and NF kappa B after 12 h which became significant after 24 h. Serum TNF-alpha and retinochoroidal tissue NF kappa B levels were similar to control animals at the 24th h of the study. Eritoran significantly reversed histopathological damage after 24 h. Conclusions. Eritoran treatment resulted in less inflammatory damage in terms of serum and retinochoroidal tissue parameters.