ANKARA INTERNATIONAL CONGRESS ON SCIENTIFIC RESEARCH-IX, Ankara, Türkiye, 26 - 28 Aralık 2023, cilt.1, sa.1, ss.1115-1120
Grain legumes (Fabaceae) have a lot of uses in human nutrition owing to their protein-rich
seeds. To enhance grain legume production and yield per unit area, new varieties are needed
to developed and spread. Under such conditions, grain legumes have a significant potential to
adopt biotechnological approaches for improving grain legumes, such as tissue culture.
Fabaceae species are difficult to regenerate in vitro, are mainly recalcitrant and frequently
exhibit high genotypic specificity. Grain legumes have lower regeneration potential than
forage legumes. Plant regeneration from somatic cell in vitro culture has proven challenging
in large seeded leguminous species, as opposed to forage legumes. Morphogenesis is
exceedingly slow in them, and associated concerns such as the production of albinos, vitreous
tissues, and no-response in dedifferentiated calli pose major challenges to the employment of
in vitro techniques in legume improvement. Within those species, the lentil was widely
studied in tissue culture experiments to conduct an efficient propagation and breeding
methods. There has a significant potential to adopt biotechnological technologies for
improving and lentils, such as tissue culture. However, due to its recalcitrant disposition in in
vitro environment, cultivar improving through biotechnology approaches appears to be
limited and difficult to regenerate. Numerous prior research have been conducted in order to
construct successful tissue regeneration systems in vitro conditions and to investigate the
possibilities of adopting biotechnology to lentil development initiatives. In this study, in vitro
regeneration protocols and difficulties of lentil plant have been investigated.