Creative Commons License

Küplemez H., Yıldırım M. U., Sarıhan E. O.

ANKARA INTERNATIONAL CONGRESS ON SCIENTIFIC RESEARCH-IX, Ankara, Turkey, 26 - 28 December 2023, vol.1, no.1, pp.1115-1120

  • Publication Type: Conference Paper / Full Text
  • Volume: 1
  • City: Ankara
  • Country: Turkey
  • Page Numbers: pp.1115-1120
  • Recep Tayyip Erdoğan University Affiliated: Yes


Grain legumes (Fabaceae) have a lot of uses in human nutrition owing to their protein-rich

seeds. To enhance grain legume production and yield per unit area, new varieties are needed

to developed and spread. Under such conditions, grain legumes have a significant potential to

adopt biotechnological approaches for improving grain legumes, such as tissue culture.

Fabaceae species are difficult to regenerate in vitro, are mainly recalcitrant and frequently

exhibit high genotypic specificity. Grain legumes have lower regeneration potential than

forage legumes. Plant regeneration from somatic cell in vitro culture has proven challenging

in large seeded leguminous species, as opposed to forage legumes. Morphogenesis is

exceedingly slow in them, and associated concerns such as the production of albinos, vitreous

tissues, and no-response in dedifferentiated calli pose major challenges to the employment of

in vitro techniques in legume improvement. Within those species, the lentil was widely

studied in tissue culture experiments to conduct an efficient propagation and breeding

methods. There has a significant potential to adopt biotechnological technologies for

improving and lentils, such as tissue culture. However, due to its recalcitrant disposition in in

vitro environment, cultivar improving through biotechnology approaches appears to be

limited and difficult to regenerate. Numerous prior research have been conducted in order to

construct successful tissue regeneration systems in vitro conditions and to investigate the

possibilities of adopting biotechnology to lentil development initiatives. In this study, in vitro

regeneration protocols and difficulties of lentil plant have been investigated.