Nationwide study of Escherichia coli producing extended-spectrum beta-lactamases TEM, SHV and CTX-M in Turkey

Cıcek A. C., Saral A., Duzgun A. O., Yasar E., Çizmeci Z., Balcı P. O., ...Daha Fazla

JOURNAL OF ANTIBIOTICS, cilt.66, sa.11, ss.647-650, 2013 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 66 Sayı: 11
  • Basım Tarihi: 2013
  • Doi Numarası: 10.1038/ja.2013.72
  • Dergi Adı: JOURNAL OF ANTIBIOTICS
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.647-650
  • Anahtar Kelimeler: beta-lactamase, CTX-M1, CTX-M2, SHV-11, TEM-1b, ESBL TYPES, ENTEROBACTERIACEAE, RESISTANCE, STRAINS, DISSEMINATION, EPIDEMIOLOGY, BLA(CTX-M), HOSPITALS, COMMUNITY, GENES
  • Recep Tayyip Erdoğan Üniversitesi Adresli: Evet

Özet

Four hundred and forty extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates were collected from 10 different hospitals in Turkey between 2011 and 2012. Clinical specimens consisted of urine (80.45%), blood (6.59%), cerebrospinal fluid (1.13%), pleural fluid (2.95%), wound (4.31%) and sputum (4.54%). ESBL-coding genes (CTX-M1, CTX-M2, TEM, SHV) were detected by PCR. According to the PCR and sequencing results, CTX-M1 was the most prevalent beta-lactamase 83.18% (366/440), followed by TEM 44.09% (194/440), CTX-M2 31.81% (140/440) and SHV 1.81% (8/440). Sequencing results showed that TEM and SHV types were TEM-1b and SHV-11, respectively. Rate of the strains harboring only CTX-M1, CTX-M2,TEM-1b and SHV-11 were 30.90%, 3.63%, 2.27% and 0.23%, respectively. Rate of the strains harboring the combinations of CTX-M1-CTX-M2, CTX-M1-CTX-M2-TEM-1b, CTX-M2-TEM-1b, CTX-M1-TEM-1b, CTX-M1-CTX-M2-TEM-1b-SHV-11, CTX-M1-TEM-1b-SHV-11, CTX-M1-SHV-11, CTX-M1-CTX-M2-SHV-11, CTX-M2-SHV-11, CTX-M2-TEM-1b-SHV-11, TEM-1b-SHV-11 were 12.95%, 11.59%, 2.95%, 26.13%, 0.45%, 0.68%, 0.22%, 0.22%, 0%, 0% and 0%, respectively. This is a nationwide study of ESBL-producing E. coli in Turkey. These results shows that CTX-M1 group is the most common type of class A beta-lactamases among ESBL-producing E. coli strains in Turkey.