The genus Scorzonera L. (Asteracea) is represented by fifty two species in Turkey. These species are being used in the treatment of several diseases due to their richness in secondary metabolites. In this study, endemic genus Scorzonera ahmet-duranii sp. nov. which is found in the South West Toros of Turkey was used as the experimental material. The species is only known in its type locality, and its population can be seen in a restricted area within the forest. For this reason, the continuity of these species is under threat. In addition, it is known that the species of the genus Scorzonera L. medicinal plants - due to their secondary metabolites - be used in the treatment of certain diseases among humans. So far, in vitro organogenesis of species of this genus has not been reported. In this study, in vitro organogenesis of S. ahmet-duranii is aimed for study. For this purpose, modified MS medium containing various concentrations of Kinetin and Naphthalene acetic acid (NAA) was used. In terms of percentage of callus formation, the most successful explant source is hypocotyl, documented as 90%. After the cultivation of apical meristems, cotyledon and hypocotyl explants in modified MS medium, shoot formation via organogenesis was observed.