Akademik Veri Yönetim Sistemi
ACTA VETERINARIA HUNGARICA, cilt.69, sa.3, ss.223-233, 2022 (SCI-Expanded)
The
aim of this study was to molecular analyze the antimicrobial resistance in enteric
bacteria from cloacal
swabs of a frog species, Pelophylax sp. regarded as an indicator
for environmental pollution. Fifty-four frog individuals were captured from six
provinces (Samsun, Ordu, Giresun, Trabzon, Rize and Artvin) in the Eastern
Blacksea Region of Turkey. One hundred and sixty bacterial colonies from the cloacal swab cultures were randomly picked, and the bacterial species were identified
by biochemical tests, automated systems (VITEK 2) and matrix assisted laser desorption
ionization time of flight mass spectrometry (MALDI-TOF-MS). Fifteen different
genera were identified in 160 strains. Antimicrobial sensitivity tests were
performed against 12 different antibiotics by disk diffusion method. The
highest rate of resistance was to ampicillin and cefazoline while the lowest
rate was against chloramphenicol and tetracycline in the bacterial species
individually. Class 1 and/or class 2 integron gene cassettes were screened by
PCR method. One of the strains (E. coli,
S1C2) was positive for intI1 gene,
which lacked a gene cassette, indicating an empty class 1 integron. In the
resistance gene analysis eight strains had blaTEM
and, four were positive for blaSHV.
tetA gene was found in two
tetracycline resistant strains. In one Klebsiella
strain (Klebsiella pneumoniae A1B1)
of three quinolone-resistant strains, the Ser83Thr substitution of the gyrA gene was detected. In addition,
Glu84Val mutation was detected in the parC
gene in two Klebsiella strains (Klebsiella pneumoniae A1B1, Klebsiella oxytoca G1C3). No
plasmid-mediated quinolone resistance genes were detected. The clonal relations
between E. coli isolates from
different locations were examined by pulsed-field gel electrophoresis. Detection
of the presence of class 1 integron in a strain of multi drug resistant enteric
bacteria in the intestinal microbiota of a cosmopolitan frog species indicates
that these animals might be a reservoire for the resistance genes, and
therefore their potential for the transfer of resistance genes to human
pathogenic bacteria can create a risk for public health.