Investigation of Total Phenolic Content and Antioxidant Activity of Guelder Rose (Viburnum opulus) Natural Grown in The Flora of Trabzon


Yurteri E. , Küplemez H. , Bahram A. K. , Özcan Aykutlu A. , Seyis F.

29th of October (Republic Day) Symposium on Scientific Researches-2, 29 - 30 Ekim 2020, ss.164-165

  • Sayfa Sayıları: ss.164-165

Özet

Guelder rose (Viburnum opulus L.) is a perennial plant belonging to the Caprifoliaceae (Honeysuckle) family. The flowers and fruit parts of guelder rose, known as gili among the right in Trabzon, have been used in the treatment of various diseases (mild asthma, epileptic seizures, high blood pressure, menstrual pains, vascular and muscle contractions, sleep disorders and nervous disorders) in European countries since ancient times and have come to the present day. The plant material of our study consisted of different parts (stem, fruit, leaf) of the Viburnum opulus L. plant collected from different altitudes (1210 m, 1220 m, 1280 m, 1380 m) from Trabzon flora. Dried and fresh fruits of guelder rose were extracted using water and 70% methanol as solvent. Total phenols in the extracts were calculated as equivalent to gallic acid (GAE) using Folin-Ciocalteu method. The total phenol content of the plant was as follows; it ranged from 86.395 mg GAE / g to 124.792 mg GAE / g. When the different parts of the plant are examined, the values of the stem, fruit and leaf were determined respectively as 107.451 - 116.122 mg GAE / gr / 86.395 - 124.173 mg GAE / gr / 117.360 - 124.792 mg GAE / gr. Looking at the results; The highest total phenol amount was reached in the leaf part (124.792 mg GAE / gr) at 1220 m altitude and the lowest in the fruit part (86.395 mg GAE / g) at 1280 maltitude. According to the DPPH radical scavenging method, the antioxidant activity content varied between 81.69-94.482%. % Inhibition values indicating the amount of antioxidant activity was determined in the stem part as 91.925-94.482 %, in the fruit part as 91.790-94.213 %, in the leaf part as 81.696-92.463 %. The highest antioxidant activity was determined in the stem part (94.482%) at an altitude of 1220 m, and the lowest antioxidant activity in the leaf part (81.696%) at an altitude of 1220 m. This study supported by TUBITAK 2209A project.