Screening Antiproliferative and Antimicrobial Effects of Ethyl Acetate Extract Driven from Camellia sinensis Flowers

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Ekşi S., Ejder N., Üreyen Ü. Z., Yazıcı Z. A., Uzunok B., Beriş F. Ş.

JOURNAL OF PHARMACEUTICS AND DRUG RESEARCH, vol.3, no.2, pp.319-328, 2020 (Peer-Reviewed Journal)

  • Publication Type: Article / Article
  • Volume: 3 Issue: 2
  • Publication Date: 2020
  • Journal Indexes: Other Indexes
  • Page Numbers: pp.319-328
  • Recep Tayyip Erdoğan University Affiliated: Yes


Plants have been used to prevent or to treat some diseases for thousand of years. As resistance to antibiotic and cancer drugs has been increasing rapidly in recent years, the scientific world has turned its focus on plants used in traditional medicine to counteract this trend. Studies conducted with the leaves of Camellia sinensis have reported that some components in the contents of the tea plant may have varying degrees of antimicrobial and anti-cancer activities. The antiproliferative effects of ethyl acetate (EtOAc) extract obtained from the flowers against non-transformed and transformed cells were evaluated with the MTT (3-[4,5-Dimethylthiazole-2-yl]-2, 5-diphenyltetrazolium bromide) assay. Additionally, the extract’s antimicrobial activity against some gram- positive (Gr+), Gram-negative (Gr-) bacteria and Candida spp. was investigated by an agar well diffusion assay and minimum inhibition concentration (MIC) was carried out using the broth microdilution method. The EtOAc extract at 200 µg/ml concentration exhibited growth ingibitory effects on all cancer cells, but had no effect on non-transformed cells. At this concentration, the inhibitory effect on cancer cells was statistically significant (p<0.05) in comparison to non-transformed ARPE-19 and HEL299 cell lines. However, the anti-proliferative effect at 100 µg/ml was retained against endometrial cancer cell line (CRL-2923) only. Notably, CRL-2923 cell line was found to be susceptible to the extract. The extract produced inhibition zones ranging from 10.0 mm to 17.3 mm for the bacteria and from 19.0 to 21.33 for yeast. Two Gr(+) and two Gr(-) bacteria were susceptible to the extract, while the other microorganisms that were used in the study were not affected. In particular, the most susceptible Gr (+) and Gr (-) bacteria were Staphylococcus aureus and Klebsiella pneumoniae with MIC values of 62.25 µg/ml and 500 µg/ml, respectively. Additionally, the extract exhibited a good antifungi activity against both Candida species with the same MIC value. Keywords: Cancer, Antimicrobial, Camellia sinensis, ARPE-19, HEL299 A549, CRL-2923, HeLa