Amsacta moorei entomopoxvirus encodes a functional DNA photolyase (AMV025)

Nalçacıoğlu R., Dizman Y., Vlak J. M., DEMİRBAĞ Z., van Oers M. M.

JOURNAL OF INVERTEBRATE PATHOLOGY, cilt.105, sa.3, ss.363-365, 2010 (SCI-Expanded) identifier identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 105 Sayı: 3
  • Basım Tarihi: 2010
  • Doi Numarası: 10.1016/j.jip.2010.06.013
  • Dergi Adı: JOURNAL OF INVERTEBRATE PATHOLOGY
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Sayfa Sayıları: ss.363-365
  • Recep Tayyip Erdoğan Üniversitesi Adresli: Evet

Özet

The major damage induced in DNA by ultraviolet light is the induction of cyclobutane pyrimidine dimers (CPDs). Amsacta moorei entomopoxvirus (AMEV) encodes a CPD photolyase (AMV025) with a putative role in converting these dimers back into monomers. In infected Lymantria dispar cells transcription of the AMV025 gene started 8 h post inoculation (p.i.) and continued through 38 h p.i. Transcription was inhibited by a DNA synthesis blocker. Transient expression in an Escherichia coli strain that lacks its endogenous photolyase, rescued growth of the UV-irradiated bacteria in a light-dependent manner, showing that AMV025 encodes a functional DNA photolyase. (C) 2010 Elsevier Inc. All rights reserved.

The major damage induced in DNA by ultraviolet light is the induction of cyclobutane pyrimidine dimers (CPDs). Amsacta moorei entomopoxvirus (AMEV) encodes a CPD photolyase (AMV025) with a putative role in converting these dimers back into monomers. In infected Lymantria dispar cells transcription of the AMV025 gene started 8 h post inoculation (p.i.) and continued through 38 h p.i. Transcription was inhibited by a DNA synthesis blocker. Transient expression in an Escherichia coli strain that lacks its endogenous photolyase, rescued growth of the UV-irradiated bacteria in a light-dependent manner, showing that AMV025 encodes a functional DNA photolyase.