Amsacta moorei entomopoxvirus encodes a functional DNA photolyase (AMV025)

Nalçacıoğlu R., Dizman Y., Vlak J. M., DEMİRBAĞ Z., van Oers M. M.

JOURNAL OF INVERTEBRATE PATHOLOGY, vol.105, no.3, pp.363-365, 2010 (SCI-Expanded) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 105 Issue: 3
  • Publication Date: 2010
  • Doi Number: 10.1016/j.jip.2010.06.013
  • Journal Name: JOURNAL OF INVERTEBRATE PATHOLOGY
  • Journal Indexes: Science Citation Index Expanded (SCI-EXPANDED), Scopus
  • Page Numbers: pp.363-365
  • Recep Tayyip Erdoğan University Affiliated: Yes

Abstract

The major damage induced in DNA by ultraviolet light is the induction of cyclobutane pyrimidine dimers (CPDs). Amsacta moorei entomopoxvirus (AMEV) encodes a CPD photolyase (AMV025) with a putative role in converting these dimers back into monomers. In infected Lymantria dispar cells transcription of the AMV025 gene started 8 h post inoculation (p.i.) and continued through 38 h p.i. Transcription was inhibited by a DNA synthesis blocker. Transient expression in an Escherichia coli strain that lacks its endogenous photolyase, rescued growth of the UV-irradiated bacteria in a light-dependent manner, showing that AMV025 encodes a functional DNA photolyase.

The major damage induced in DNA by ultraviolet light is the induction of cyclobutane pyrimidine dimers (CPDs). Amsacta moorei entomopoxvirus (AMEV) encodes a CPD photolyase (AMV025) with a putative role in converting these dimers back into monomers. In infected Lymantria dispar cells transcription of the AMV025 gene started 8 h post inoculation (p.i.) and continued through 38 h p.i. Transcription was inhibited by a DNA synthesis blocker. Transient expression in an Escherichia coli strain that lacks its endogenous photolyase, rescued growth of the UV-irradiated bacteria in a light-dependent manner, showing that AMV025 encodes a functional DNA photolyase. (C) 2010 Elsevier Inc. All rights reserved.