Nowadays; free electromagnetic field (EMF) is quite high in outdoor with the discovery of novel devices. This sources leads proliferation of free radicals in intracellular area by means of reactive oksigen species (ROS). Melatoin and omega-3 (ω-3) are two well-known antioxidative agents in recent literature. The aim of our study was to investigate the celular effects of 900 MHz EMF exposure and neuroporective efficiency of Mel and w-3 on damaged granular cells in dentate gyrus of adult rats. For this purpose; selected animals were randomly divided into four equal groups (n=6). The Cont group that was not exposed to any procedure; the EMF group, exposed to 900 MHz electromagnetic field for 1h/day group; the EMF+Mel group, 50 mg/kg/day Mel (Sigma-Aldrich Comp., St Louis, MO, USA) injected intraperitoneally (i. p.) group during 900 MHz electromagnetic field exposure time (Jahnke et al., 1999) and the EMF+ω3 group, animals fed with standard feed added Ω3 substances (Sigma Chemical Comp., St Louis, MO, USA) %10 of standard feed. Granular cells were estimated using the optical fractionator, which is one of unbiased stereological techniques, by stereoinvestigator analysing system. GSH, CAT and SOD enzyme activities have determined at respectively 410 nm, 540 nm, and 450nm. When the granular cell numbers of the groups were estimated, although a highly significant decrease in EMF group compared to other study groups (p<0.01), in terms of granular cell numbers for each group, there was no significantly difference between EMF+Mel and Cont, EMF+ω3 and Cont, EMF and Cont (p> 0.05). Similarly, there was no significantly difference between EMF+ω3 and EMF+Mel groups. Also, the activities of CAT, total GSH, and SOD in serum among EMF, Cont, EMF+Mel, EMF+ω3 (A-C) was shown by using spectrophotometer. There was no significantly difference between Cont and EMF+ω3 in terms of CAT and total GSH activities. Additionally, Serum SOD activity did not show statistically significant differences among the Cont, EMF+Mel, and EMF+ω3 (p> 0.05). There was highly significant increase in serum enzyme activities of EMF compared to other study groups (p <0.01). When examined the serum CAT and total GSH activities of EMF+Mel, there was highly significant increase in comparison with Cont and EMF+ω3 (p<0.01). In conclusion, EMF reduced number of granuler cells in dentate gyrus. Using Mel and ω-3 have neuroprotective effect if used after EMF exposure.