Deletion of the hfsB gene increases ethanol production in Thermoanaerobacterium saccharolyticum and several other thermophilic anaerobic bacteria

Eminoğlu A. , Murphy S. J. , Maloney M., Lanahan A., Giannone R. J. , Hettich R. L. , ...More

BIOTECHNOLOGY FOR BIOFUELS, vol.10, 2017 (Journal Indexed in SCI) identifier identifier identifier

  • Publication Type: Article / Article
  • Volume: 10
  • Publication Date: 2017
  • Doi Number: 10.1186/s13068-017-0968-9
  • Keywords: Thermoanaerobacterium saccharolyticum, Clostridium thermocellum, Thermoanaerobacter mathranii, Thermoanaerobacterium thermosaccharolyticum, Thermoanaerobacterium xylanolyticum, Hydrogenase, Ethanol, CLOSTRIDIUM-THERMOCELLUM, BIFUNCTIONAL ALCOHOL, RALSTONIA-EUTROPHA, REX, TRANSFORMATION, HYDROGENASE, EXPRESSION, REPRESSOR, SENSOR, STRAIN


Background: With the discovery of interspecies hydrogen transfer in the late 1960s (Bryant et al. in Arch Microbiol 59:20-31, 1967), it was shown that reducing the partial pressure of hydrogen could cause mixed acid fermenting organisms to produce acetate at the expense of ethanol. Hydrogen and ethanol are both more reduced than glucose. Thus there is a tradeoff between production of these compounds imposed by electron balancing requirements; however, the mechanism is not fully known.