The comparison of persimmon genotypes (Diospyros kaki Thunb.) by using RAPD and FAME data


Akbulut M. , ERCİŞLİ S., Yildirim N., ORHAN E., AĞAR G.

Romanian Biotechnological Letters, vol.13, no.4, pp.3851-3858, 2008 (Journal Indexed in SCI) identifier identifier

  • Publication Type: Article / Article
  • Volume: 13 Issue: 4
  • Publication Date: 2008
  • Title of Journal : Romanian Biotechnological Letters
  • Page Numbers: pp.3851-3858

Abstract

In the current study, FAME (Fatty acid methyl esters) and RAPD profiles were used to examine biochemical and genetic relationships between 31 selected persimmon genotypes sampled from Persimmon Repository, Black Sea Agricultural Research Institute, Samsun, Turkey. Fatty acid composition of persimmon leaves was determined by using gas chromatography. The dendrogram realized from the RAPD markers grouped the 31 genotypes into three major clusters. Cluster 1 included 8, cluster 2 included 14 and cluster 3 included 9 genotypes. The fatty acid results showed that there were differences among persimmon genotypes both percent and presence of fatty acids in leaves. Some relationships between RAPD and FAME data were found. The linolenic acid was detected only in genotypes in cluster 3. Behenic acid was detected only in genotypes within cluster 1. According to these results, it can be concluded that the use of RAPD and FAME data could be useful indicator for characterization and grouping of persimmon genotypes. Copyright © 2008 Bucharest University.