An efficient method for maintaining clarity and forming tight and low amount of sediment in pomegranate juice during storage: Enzymatic hydrolysis with “tannase + lactonase + papain”

TÜRKYILMAZ M., HAMZAOĞLU F., ÖZKAN M.

Food Chemistry, cilt.480, 2025 (SCI-Expanded) identifier identifier

  • Yayın Türü: Makale / Tam Makale
  • Cilt numarası: 480
  • Basım Tarihi: 2025
  • Doi Numarası: 10.1016/j.foodchem.2025.143941
  • Dergi Adı: Food Chemistry
  • Derginin Tarandığı İndeksler: Science Citation Index Expanded (SCI-EXPANDED), Scopus, Academic Search Premier, PASCAL, Aerospace Database, Aquatic Science & Fisheries Abstracts (ASFA), BIOSIS, CAB Abstracts, Chemical Abstracts Core, Chimica, Communication Abstracts, Compendex, EMBASE, Food Science & Technology Abstracts, Metadex, Veterinary Science Database, Civil Engineering Abstracts
  • Anahtar Kelimeler: HHDP-galloyl glucuronide, Prolamin, Punicalagin-2, Sediment, Turbidity
  • Recep Tayyip Erdoğan Üniversitesi Adresli: Evet

Özet

Turbidity and sediment formation in pomegranate juice (PJ) is a consequence of the interaction between tannins and proteins. The aim of this study was to disrupt this interaction by enzymatic hydrolysis of tannins [“tannase,” “lactonase (R-PON-1)”] and proteins (papain). The effect of enzymatic treatments on sediment/turbidity was studied throughout storage (20 °C). Low and tight sediment formation was only possible when protein and tannins were hydrolysed together. In “tannase + R-PON-1 + papain” treated PJ, turbidity decreased after storage and the lowest and tightest sediment was also detected (p < 0.05). The strongest correlations were found between the “prolamin/punicalagin-2” ratio and turbidity (r = 0.997) and tight sediment formation (r = −0.964). This ratio should be 4.38 ± 0.07 to completely eliminate sediment formation in PJ. To achieve this ratio, punicalagin-2 should be added to PJ. This study is the first to show that punicalagin-2 should be added to PJ to produce a clear and sediment-free PJ, contrary to previous clarification methods.