Polyphenol oxidase properties, anti-urease, and anti-acetylcholinesterase activity of Diospyros lotus L. (Plum Persimmon)


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Faiz O. , BALTAS N.

INTERNATIONAL JOURNAL OF FOOD PROPERTIES, cilt.20, sa.5, ss.1186-1196, 2017 (SCI İndekslerine Giren Dergi) identifier identifier

  • Cilt numarası: 20 Konu: 5
  • Basım Tarihi: 2017
  • Doi Numarası: 10.1080/10942912.2016.1205086
  • Dergi Adı: INTERNATIONAL JOURNAL OF FOOD PROPERTIES
  • Sayfa Sayıları: ss.1186-1196

Özet

Diospyros lotus fruit polyphenol oxidase was purified using affinity chromatography, resulting in a 15-fold enrichment in specific activity. The purified enzyme, having 16.5 kDa molecular weight on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, exhibited the highest activity toward 4-methylcatechol. Maximum diphenolase activity was reached at pH 7.0 and 60 degrees C in the presence of 4-methylcatechol. K-m and V-max values were calculated as 3.8 mM and 1250 U/mg protein, respectively. Ascorbic acid was a promising inhibitor with an IC50 value of 0.121 mu M. The activity of the purified enzyme was stimulated by Fe2+, Sr2+, Zn2+, and K+ and deeply inhibited by Hg2+, at 1 mM final concentration. Aqueous extract of Diospyros lotus L. fruit showed strong substantial urease and acetylcholinesterase inhibition, with IC50 values of 1.55 +/- 0.05 and 16.75 +/- 0.11 mg/mL, respectively.