In this study, the effect of the depuration period on proximate composition, metals, fatty acids, and bacteria in the edible tissue of warty venus (Venus verrucosa) collected from İzmir Bay in the Aegean Sea was investigated. In addition, benefit and risk for human health associated with the consumption of warty venus were determined by atherogenic index (AI), thrombogenic index (TI), polyene index (PI), fish lipid quality (FLQ), target hazard quotient (THQ), and hazard index (HI). The depuration process did not show a significant (P > .05) effect on proximate composition, except for ash. The depuration process was successful in reducing bacteria growth and the concentrations of all metals, except for copper. Total aerobic mesophilic bacteria count of 1.94 log CFU/g in the fresh samples was uncountable at 24 h of depuration. The concentrations of cadmium, lead, copper, iron, and zinc were below the limit values allowed for safe consumption. The analyzed metals were safe for human consumption based on provisional tolerable weekly intake (PTWI), THQ, and HI. Significant variations were also observed in the fatty acid values depending on the depuration periods (P < .05). The n3/n6 and polyunsaturated/saturated fatty acid ratios were greater than those recommended by the FAO/WHO. Moreover, the AI and TI of samples were under established limits.